One technology that deserves greater attention from both
the public and policymakers is the use of tissue culture, the
most widely used application of which involves creating copies
of plants through a process known as micropropagation.
In essence, micropropagation involves taking tissue (known
as an 'explant') from a plant and growing it on sterile media
containing substances essential for growth. Once it is growing
well, samples of this culture can be taken and used to grow
entire plants under laboratory conditions.
The technique is currently used mainly with perennial crops
that can reproduce vegetatively, producing new stems directly
from the existing ones rather than needing to be pollinated
and produce seeds.
It can be used to create millions of new 'clones' from a single
plant, each genetically-identical to the parent plant.
The method can be used to produce large quantities of high-quality
plant lines, to eliminate pathogens from infected planting
materials, or to produce 'true-to-type' material from desirable
plant lines.
Micropropagation has been developed over many decades, and
can now be considered a 'mature' plant biotechnology. It is
already widely used in developing countries, especially Asia
- in particular as a result of the immense market in China
for plants generated in this way.
It is relatively cheap, and has been shown in general to increase
productivity (especially of root and tuber crops, such as sweet
potatoes and potatoes).
Its most common application in developing countries involves
producing virus-free plantlets by heat-treating the explant
to kill any viruses present and then culturing cells from its
'meristem', the plant's actively growing tissue.
Because micropropagation cannot, however, guarantee that plants
will be virus-free access to a virus diagnostic facility is
essential.
Anther culture and embryo rescue
Another widely used tissue culture technique, 'anther culture',
uses the immature pollen-producing organs of a plant to generate
fertile 'haploid' plants, which have half the full set of genetic
material.
These plants can later be crossed to produce pure homozygous
'diploid' plants, with identical copies of each gene, thus
eliminating undesirable variation in key traits.
The technique is popular among breeders as an alternative
to the numerous cycles of inbreeding or 'backcrossing' usually
needed to obtain pure lines.
In vitro anther culture is now used routinely for improving
vegetables, such as asparagus, sweet pepper, eggplant, watermelon
and Brassica vegetables. It is also used, though to a lesser
extent, for cereal crops such as rice, barley and wheat.
A further refinement of the technique is the so-called 'microspore
culture'. This involved isolating and culturing the cells from
which pollen grains develop, and can yield up to ten times
as many haploid embryos as anther rescue.
A further tissue culture technique, known as 'embryo rescue'
(or sometimes 'embryo culture') involves crossing species that
are not normally sexually compatible. In nature embryos that
result from such 'wide crosses' usually fail to develop. But
in the laboratory, wide crosses can be used to transfer genetic
traits from wild relatives of crops (i.e. secondary and tertiary
gene pools) into cultivated crop plants (primary gene pools).
An example is triticale, a relatively new hybrid variety that
is the result of a cross between rye and wheat.